International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 7, Issue:10, October, 2018

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(10): 2799-2806

Molecular Characterization of Trichoderma Mutants with RAPD Marker
Radhika Deshmukh, P.N. Davhale and Maya Nadare
Department of Plant Pathology, Post Graduate Institute, Dr. PDKV Akola (MS) 444104, India
*Corresponding author

Trichoderma is an important biocontrol agent of several soil borne plant pathogens. The antagonistic ability of a biocontrol agent was determined by its physiological state so that change in physiological or genetical condition could alter the antagonism. Genetic modification using mutagenesis offers the potential for producing improved bio protection is likely to enhance their biocontrol capabilities against soil borne pathogen. Molecular techniques are important analytical tool to characterized genetic variability and diagnosis of microbial population. Molecular variability of Trichoderma mother culture and its mutants was studied by using Random Amplified Polymorphic DNA (RAPD). 20 RAPD primer of OPA series were tested, of which 14 primers produced 72 scorable bands among them 52 bands were polymorphic and level of polymorphism was upto 72%. On the basis of dendrogram, the tested cultures were clearly divided into 2 groups. First group i.e. cluster A included 4 mutants TvM-5, TvM-3, TvM-1 TvM-2 and mother culture TvMC. Second group i.e. cluster B included only one mutant i.e. TvPM-4.

Keywords: Trichoderma mutants, DNA extraction, RAPD marker, Scorable bands

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How to cite this article:

Radhika Deshmukh, P.N. Davhale and Maya Nadare. 2018. Molecular Characterization of Trichoderma Mutants with RAPD Marker.Int.J.Curr.Microbiol.App.Sci. 7(10): 2799-2806. doi:
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.