Two hundred and forty, day old chicks were distributed randomly into four treatments with three replicates in each treatment (20 birds in each replicate) and were fed standard feed. The trial was studied for 42 days which comprised of four dietary treatments wherein T1 served as basal diet. Basal diet+ 250g/ton (AGP), basal diet + Addon Poultry Max (250g/ton) and basal diet + Probiotics (Addon Poultry Max) (250g/ton) and Enrofloxacin (10ml/100kg BW) levels were designated as T2, T3 and T4 respectively. Effects of different treatment groups on serum biochemical (total protein, cholesterol) parameters and immune response (CMI to PHA-P, HI to ND vaccine) were evaluated. The humoral immune response to SRBC indicated insignificant (P>0.05) influence, while CMI response with P-HAP was significantly (P<0.05) higher in broilers raised on Probiotics (Addon Poultry Max) supplemented groups compared to the control group. Concentration of serum cholesterol was not affected due to treatments. However, numerically lower total serum cholesterol values were observed in Probiotics (Addon Poultry Max) supplemented when compared to control group. The highest serum total protein value was observed with Probiotic (Addon Poultry Max) (5.49 g/100ml) followed by T4 group (4.95 g/100ml) during sixth week. Hence, it can be concluded that, supplementation of Probiotic (Addon Poultry Max) as alternative to antibiotic can be used for improving performance of broiler chicken.

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