Cytomegalovirus infections are endemic worldwide. The most frequently used methods for detecting antibodies in developing world are the Enzyme Linked Immunosorbent Assay. The polymerase chain reaction is a molecular biology technique in which the production of large amounts of specific deoxyribonucleic acid fragments is induced from very low concentrations of complex substrates allowing the detection of very low amounts of viral particles. Blood samples were collected from pregnant women with bad obstetric history attending Outpatient Department and were stored at -20°C and transported to the main Laboratory of Department of Microbiology where DNA was extracted from the serum and PCR was performed for CMV DNA amplification. A total of 47 women were screened, amongst them, 4 (8.5%) were cytomegalovirus (CMV) DNA positive by polymerase chain reaction (PCR) whereas 47 Blood donors were screened, amongst them, 1 (2.1%) were cytomegalovirus (CMV) DNA positive. Prevalence was recorded in age group 21 to 30 years. The detection of HCMV in women before pregnant should reduce miscarriage rate and also the number of congenitally infected infant. Real-time PCR is the best technique and more sensitive and effect than conventional PCR and ELISA.

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