Patient safety has become a priority in healthcare system and patient safety culture among health care workers is a driving force for its application. To assess patient safety culture dimensions at work unit level among HCWs in Tanta University Hospitals. A cross-sectional study was conducted over a period of 1 year using Hospital Survey on Patient Safety Culture of AHRQ. The dimensions with the highest positive responses were; teamwork within hospital units, organizational learning-continuous improvement, and supervisors/ manager expectations and actions promoting safety (69.7%, 65.8%, and 60.8%, respectively) and the least positive response was in the dimension of non-punitive response to error (22.7%). Non-punitive work environment is essential to facilitate change in patient safety culture where blameless reporting helps better detection and management of adverse events.

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