National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Variability in 20 isolates of Rhizoctonia solani Kühn [Teleomorph: Thanatephorus cucumeris (Frank) Donk] causing sheath blight of rice and weeds, was observed by RAPD and ISSR DNA analysis in conjunction with morphology and pathogenicity studies. Studies on morphological characterization of R. solani isolates showed that isolates were highly variable in mycelial growth, color and sclerotial parameters. Pathogenicity of R. solani was also evaluated and based on their virulence, isolates were classified highly virulent. Genetic diversity of R. solani isolates from different locations using three RAPD and ISSR markers showed less polymorphism at DNA level. The PIC value ranged from 0.82 to 0.88. Relation between cultural/morphological characteristics, Pathogenicity and genetic variation based on RAPD and ISSR markers, and possible reasons for high intra-group variability are discussed.
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