In the present investigation, 235 quarter milk samples from 59 apparently healthy lactating buffaloes were collected to compare different diagnostic tests for detection of Sub-Clinical Mastitis (SCM) in Buffaloes. Out of 235 quarter milk samples, 17.02 per cent, 16.59 per cent, 16.17 per cent, 14.49 per cent, 14.46 per cent and 17.02 per cent quarters were tested positive for SCM by bromothymol blue (BTB) strip test, electrical conductivity (EC), California mastitis test (CMT), N-acetyl-β-D-glucosaminidase (NAG-ase), Somatic cell count (SCC) and Cultural Examination, respectively. While calculating percent sensitivity, specificity and predictive value of these tests taking cultural examination as standard, the NAG-ase test was found to have highest sensitivity (60%), Specificity (94.87%) and predictive value (70.58%) followed by SCC, CMT, EC and BTB strip test, respectively. NAG-ase test can be used as a preferential laboratory tool over SCC and cultural examination, however CMT can be used as a first screening tool for detecting suspected samples for further investigation for SCM.

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