National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Pathogenic Escherichia coli strain from faeces of sloth bears was screened by using culture morphology, Gram’s staining, biochemical tests and polymerase chain reaction. Our results showed that out of 60 samples collected, 59 samples (98.33%) were cultured on EMB agar and Gram’s stain revealed rod-shaped Gram-negative organism with green metallic sheen - like colonies. The biochemical tests of cultured samples revealed positive to indole production, positive to methyl red test, negative to Voges-Proskauer test, negative to Simmon citrate utilization test, positive to glucose, negative to H2S production and negative to urea production. All E. coli isolates were found sensitive to azithromycin, streptomycin and tetracycline. They were found intermediate to enrofloxacin and gentamicin. The fimC gene was amplified by PCR for the pathogenic Escherichia coli and were found to be positive of 53.33% among the juveniles, 46.67% among the sub-adults, 53.33% among the adults and 33.33% among the geriatrics were found to be positive. This study may provide information for developing strategies in the future in the control of Escherichia coli infections in sloth bears.
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