An experiment was conducted to evaluate the levels of exogenous fibrolytic enzymes (EFE) on in vitro digestibility of dry matter (DM) and organic matter (OM), net gas production (NGP), metabolizable energy (ME) content, microbial biomass production (MBP) and efficiency of microbial biomass production (EMP). The total mixed ration (TMR) was prepared using sorghum hay and groundnut straw and compound concentrate mixture @ 30, 30 and 40%, respectively, to meet nutrient requirement of cow (500 kg) producing 12 kg fat corrected milk. The cellulase and xylanase was incorporated @ 0+0, 1000+2000, 1000+3000, 1000+4000, 2000+4000, 2000+6000, 2000+8000, 3000+6000, 3000+9000, 3000+12000, 4000+8000, 4000+12000, 4000+16000, 5000+10000, 5000+15000, 5000+20000, 6000+12000, 6000+18000 and 6000+24000 IU/kg TMR. The TMR with different levels of cellulase and xylanase were in vitro incubated to ascertain their effect on digestibility, gas production, and nutritive values. The significantly (p<0.05) higher and optimum in vitro digestibility of DM (66.243%) and OM (66.56%), NGP (76.48 ml/500 mg TMR) and ME (7.41 MJ/kg DM) were observed at 3000+12000 cellulase and xylanase IU/kg TMR supplementation, whereas MBP (146.73 mg/500 mg TMR) and EMP (44.02) were significantly (p<0.05) higher and optimum at C4X12 and C5X15 TMR, respectively. The MBP (145.86 mg/500 mg TMR) and EMP (43.83) also better at C3X12 TMR. The levels of cellulase 3000 + xylanase 12000 IU/kg TMR were found suitable for further in vivo study in crossbred cows.

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