National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
In present study an attempt has been made to isolate and identify the dark septate endophytic fungi (DSE) and their colonization in the roots of an important ethno-medicinal plant, Plumbago zeylanica L. The plant exhibited significant colonization by DSE. Two different strains of dark septate endophytic fungi were isolated from the roots of the plant. The pure cultures were established (PUB and PUD). DNA was isolated from the Pure culture – PUB and PUD and 28s rDNA sequencing of D1, D2 region of the larger sub unit was performed. Sequence analysis exhibited sequence similarity with Perenniporia sp. CBS 130020 (Gen Bank Accession Number: JX292098.1) and Aspergillus niger strain RA402 (GenBank Accession Number: GQ169753.1) respectively based on nucleotide homology and phylogenetic analysis.
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