National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Bacterial blight (BB) and rice blast are the two most important diseases causing significant yield loss in rice, and they are endemic to several rice growing states of India. In Telangana and Andhra Pradesh states, the yield loss is very severe due to BB and blast. In order to address the above said issues, the efforts have been made by Institute of Biotechnology, Rajendranagar, Hyderabad, and developed MTU1010 and Tellahamsa introgression lines with bacterial blight (xa13 and Xa21) and blast (Pi54 and Pi1) resistance genes by backcrossing with donor parents. GPP2 was used as a donor for bacterial blight resistance genes (xa13 and Xa21), while NLR145 (Swarnamukhi) was used as donor for blast resistance genes (Pi54 and Pi1). Genotyping of these advanced backcross progenies of MTU1010 (BC4F5) and Tellahamsa (BC3F4) was carried out to check the uniformity of plants with in the progeny in homozygous condition for target genes. DNA bulking method was used to reduce the time, resources and number of samples from 3465 to 99 for DNA isolation without compromising the quality of DNA. Genotyping was carried out in 29 advanced backcross progenies (BC3F4, BC4F5) for the presence of four target genes viz., xa13, Xa21, Pi54 and Pi1 by using gene specific / linked PCR based molecular markers viz., xa13-prom, pTA248, Pi54 MAS and RM224, respectively. The bulk sample analysis of 29 progenies exhibited uniformity for the target genes.
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