National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Coagulase negative staphylococcus are the commensal of humans, which are increasingly being recognized as significant nosocomial pathogens, mainly in patients under long term medical care or in immunocompromised patients. Identification and speciation of CONS is becoming medically important due to its multidrug resistant patterns and to define its clinical significance. Study was carried out from of Dec 2014 till May 2015. A total of 60 isolates from various clinical specimen was collected in a tertiary care centre. Speciation was done by conventional method which included biochemical characterization and automated method like Vitek 2 GP 21342. Antibiotic sensitivity testing was done by Disc diffusion method on Muller Hinton Agar. Out of 60 isolates Staphylococcus haemolyticus (26%) was most frequently isolated, followed by S.cohnii (23%), S.xylosus (13%), S.lugdunensis (11%), S.epidermis (10%), S.stimulans (10%), S.saprophyticus (3%), S.schleiferi (3%) and S.warnerii (3%). This finding was based on the conventional method of speciating CONS. From the total of 60 isolates Vitek correctly identified S.hamolyticus (26%), s.cohnii(23%), S.epidermidis(10%) and S.saprophyticus (3%), for the remaining species like S.xylosus, S.lugdunensis, S.simulans, S.schleiferi and S.warnerii the result was ambiguous and correct identification was done based on the supplemental test suggested by the manufacturer, by the conventional method only 3% was misidentified to be S.warneri which the automated method idnetified to be S.epidermidis. Antibiotic sensitivity testing showed 36% of methicillin resistant CONS and 63% of methicillin sensitive CONS from the same 60 isolates. Resistances to other group of drugs were also studied. Emergence of various drug resistances makes it important for speciation of CONS and to study their Antibiogram pattern for treating the infection caused by these organisms. Though speciation of CONS by automated method is expensive but it is reliable and accurate than conventional method which is more cumbersome and variable results are obtained.
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