National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
One of the reasons for organisms being capable of defending themselves from host immune systems is their capability to form biofilms. The interior of the bacterial biofilms presents greater resistance to opsonisation by antibodies and to phagocytosis, which explains the chronic character of these infections (Parsek et al., 2003). Furthermore, the information on the capacity of a clinical isolate to produce biofilm would help a clinician to evaluate the measure of its virulence and devise an appropriate treatment plan for the patient (Christensen et al., 1985). The objective of this study is to compare the biofilm production in Staphylococcus aureus and Coagulase negative Staphylococci (CONS) using Congo red agar and Tube method and also to compare antibiogram of Biofilm producers and non-biofilm producers. Among the total sample size of 497 clinical bacterial isolates, 60 isolates were Staphylococcus, out of which 46 isolates (76%) were Staphylococcus aureus and 14 (23%) were Staphylococcus epidermidis based on morphology and biochemical properties. From 46 Staphylococcus aureus isolated, 21 (47.82%) isolates were non-biofilm producers. 25 (54.34%) isolates produced biofilm as detected by Tube Method (TM),out of which, 11 (44%) biofilm producers were detected by both the Congo Red Agar (CRA) and Tube Method (TM).14(56%) biofilm producers were detected only by tube method. Among the 14 isolates of (23%) Staphylococcus epidermidis, 4 (28%) isolates were non-biofilm producers and 10 (71%) isolates were biofilm producers as detected by Tube Method (TM). Out of which, 5 (50%) biofilm producers were detected by both Congo Red Agar (CRA) and Tube Method (TM). 5(50%) biofilm producers were detected only by tube method In our study we found that,23 (92%) biofilm producers and 13 (68.42%) non biofilm producers of Staphylococcus aureus and 7 (70%) biofilm producers and 2(50%) non biofilm producers of Staphylococcus epidermidis, were resistant to Penicillin and biofilm producers of Staphylococcus epidermidis, showed higher resistance to Ciprofloxacin and Cotrimoxazole.
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