International Journal of Current Microbiology and Applied Sciences |
ISSN: 2319-7706 Volume 2 Number 4 (2013) pp. 108-116 |
Decolorization and degradation of Azo dye - Remazol Black B by newly isolated Pseudomonas putida |
S.Kannan1
, K.Dhandayuthapani2
* and Mazher Sultana3 |
1Department of Zoology, Manonmanium Sundaranar University, Tirunalvalli, Tamil Nadu, India. 2Department of Plant Biology and Biotechnology, A.A. Govt. Arts College, Cheyyar -604 407, Tamil Nadu, India. 3Department of Advance Zoology and Biotechnology, Presidency, College, Chennai - 600 005 Tamil Nadu, India *Corresponding e-mail: kdpani@gmail.com |
A B S T R A C T |
Disposal of dyes into the environment causes serious damage and also they may be
toxic to some aquatic organisms due to their breakdown products. The chemical and
physical methods have many disadvantages which can overcome by biological method because it is cost saving and environmentally benign. Among different decolorizing microorganisms bacteria can degrade and even completely mineralize many dyes under certain conditions. In the present study an attempt was made to
examine the potential of newly isolated Pseudomonas putida for decolorization of azo
dye- Remazol Black B in batch reactor. The influence of different concentration of glucose, pH and temperature on decolorization was studied to find the optimum
conditions required for maximum decolorization and degradation. pH 7.0 and 35
0C were considered to be the optimum decolorizing conditions because in these
conditions only the maximum decolorize was found. 5g/L glucose present media
showed the maximum decolorization. This new isolate grew well in a high
concentration of dye (300mg L
-1
) and 97.12% decolorized within 48 h and also
tolerated upto 1000mg/L of dye. Colorless cells of P. putida and UV Visible spectroscopic analyses suggested that the decolorizing activity only through
biodegradation not by inactive surface adsorption. The above results show the potential of this bacterial strain to be used in the biological treatment of textile
effluent under optimum condition. |
Keywords |
Decolorization; Glucose; Pseudomonas putida; Remazol Black B; Textile effluent. |