International Journal of Current Microbiology and Applied Sciences
ISSN: 2319-7706 Volume 2 Number 4 (2013) pp. 108-116
Decolorization and degradation of Azo dye - Remazol Black B by newly isolated Pseudomonas putida
S.Kannan1 , K.Dhandayuthapani2 * and Mazher Sultana3 
1Department of Zoology, Manonmanium Sundaranar University, Tirunalvalli, Tamil Nadu, India. 2Department of Plant Biology and Biotechnology, A.A. Govt. Arts College, Cheyyar -604 407, Tamil Nadu, India. 3Department of Advance Zoology and Biotechnology, Presidency, College, Chennai - 600 005 Tamil Nadu, India *Corresponding e-mail: 
Disposal of dyes into the environment causes serious damage and also they may be toxic to some aquatic organisms due to their breakdown products. The chemical and physical methods have many disadvantages which can overcome by biological method because it is cost saving and environmentally benign. Among different decolorizing microorganisms bacteria can degrade and even completely mineralize many dyes under certain conditions. In the present study an attempt was made to examine the potential of newly isolated Pseudomonas putida for decolorization of azo dye- Remazol Black B in batch reactor. The influence of different concentration of glucose, pH and temperature on decolorization was studied to find the optimum conditions required for maximum decolorization and degradation. pH 7.0 and 35 0C were considered to be the optimum decolorizing conditions because in these conditions only the maximum decolorize was found. 5g/L glucose present media showed the maximum decolorization. This new isolate grew well in a high concentration of dye (300mg L -1 ) and 97.12% decolorized within 48 h and also tolerated upto 1000mg/L of dye. Colorless cells of P. putida and UV Visible spectroscopic analyses suggested that the decolorizing activity only through biodegradation not by inactive surface adsorption. The above results show the potential of this bacterial strain to be used in the biological treatment of textile effluent under optimum condition. 
Decolorization; Glucose; Pseudomonas putida; Remazol Black B; Textile effluent.