International Journal of Current Microbiology and Applied Sciences
ISSN: 2319-7706 Volume 2 Number 4 (2013) pp. 84-96
An Investigation of DNA Fragmentation and Morphological Changes caused by Bacteria and Fungi in Human Spermatozoa
S.Sasikumar1 *, D.Dakshayani1 and D.Sarasa2 
1Life care DKJ Hospital, Villivakkam, Chennai, Tamilnadu, India. 2Department of Zoology, Quaid-e-Millath Government College for Women, Chennai- 600 002, Tamil Nadu, India. *Corresponding author: 
The main aim of this study verifies the prevalence of semen Bacterial contamination and whether the contamination could cause the DNA fragmentation towards decreased sperm quality. Spermiogram, Toluidine blue staining, semen culture for bacteria, papanicolaus staining for sperm morphology assessment and sperm DNA fragmentation has been performed. Sperm morphology, seminal DNA fragmentation data were elaborated using a semen culture report and semen morphology and DNA fragmentation analysis able to define that semen sample as whether normal or abnormal and percentage of sperm morphology and DNA fragmentation. Patients data were divided according to the sperm DNA fragmentation by toluidine blue staining, assessment of sperm morphology by papanicolaus staining and semen for Bacteria and Fungi isolation. According to results comparison significantly sperm quality was decreased, in terms of motility, DNA defect, acrosome defect, multiple morphology defects and sperm necrosis were seen in sperm DNA fragmentation, > than 30% morphological defect, Bacteria and Fungi isolated specimen. The DNA fragmentation and morphological changes of the spermatozoa might adversely affect the human in vivo fertilization of Embryo or fertilization through the assisted reproductive techniques. 
DNA fragmentation; human spermatozoa; bacteria and fungi; Fertilization; Chlamydia trachomatis.