International Journal of Current Microbiology and Applied Sciences

ISSN: 2319-7706 Volume 2 Number 3 (2013) pp. 11 18

Effect of plant growth promoting Bacillus subtilis and Pseudomonas fluorescence as Rhizobacteria on seed quality of sorghum

K.S. Prathibha1 and K.G.Siddalingeshwara2*

1Department of Biotechnology, K.L.E s S. Nijalingappa College, Bangalore-10, India 2Environmental Health and Safety Research and Development Centre, Bangalore-10, India *Corresponding Author: siddha_lingeshwar@rediffmail.com

A B S T R A C T

Sorghum (Sorghum bicolor (L) Moench) is vital life sustaining crop in many parts of the world ranking fifthafter wheat (Tritium species), rice (Oryza spp) maize (Zea mays) and barley (Hordeum vulgare). Nutritionationally it is superior to all other cereals. Sorghum is vulnerable to many disease including 23 different fungal diseases. The plant-growth promoting rhizobacteria (PGPR) such as Pseudomonas fluorescens and Bacillus subtilis were used to study the effect on seed germination and also on nutritional qualities such as total protein, carbohydrate and also peroxidase activity. Therefore, the present study showed PGPR strains, Pseudomonas fluorescens and Bacillus subtilis were effective in improving seed quality such as seed germination, vigour index and nutritional quality such as protein content and carbohydrate content. In addition to this peroxidase activity also observed. So these PGPR can be used to improve the quality of sorghum. The molecular weight of the protein were carried out by SDSPAGE.

Keywords

Pseudomonas fluorescens; Bacillus subtilis; Sorghum seeds; SDS-PAGE; peroxidase activity.