International Journal of Current Microbiology and Applied Sciences
ISSN: 2319-7692 Volume 2 Number 2 (2013) pp.56 61

Organogenic plant regeneration via callus induction in Stevia rebaudiana Bert

V. Guruchandran and C.Sasikumar*
Department of Biotechnology, Nehru Memorial College, Puthanampatti - 621 007,
Tiruchirappalli, Tamilnadu, India
*Corresponding author:


Stevia rebaudiana is investigated as optional for artificial sweetening agents. In the present investigation an efficient and reproducible protocol for organogenesis using different explants from 3-4 months old plantlets of S.rebaudiana was studied. Maximum callus induction occurred in leaf explant cultured in the MS medium fortified with 2, 4 D (1.5mg/L) in combination with BAP (0.5mg/L).Maximum number of multiple shoots regenerated on MS medium fortified with BAP (1.5mg/L) in combination with kinetin (0.5 mg/L). BAP (1.5 mg/L) in combination with GA3 (1.0mg/ L) plays a vital role on the elongation of shoots. Half strength MS Medium in combination with NAA (1.0mg/ L) plays a vital role on rooting of shoots.

Keywords :Stevia rebaudiana; Organogenesis; Callus; Growth regulators.