International Journal of Current Microbiology and Applied Sciences
ISSN: 2319-7706 Volume 2 Number (2013) pp.44 55.

Anticancer potentials of secondary metabolites from endophytes of
Barringtonia acutangula and its molecular characterization

P. Jayashree Lakshmi and *K. Vanmathi Selvi
Department of Microbiology, Sri Akilandeswari Women s College, Vandavasi,
T.V.Malai, Tamil Nadu, India.
*Corresponding author:


Two species of Endophytic fungi (EFB01 and EFB02) were isolated from leaves of Barringtonia acutangula, a medicinal plant and secondary metabolite extraction was performed using organic solvents. The extracts were screened for anticancer assay against Human Colon Cancer cell lines HT29. Extract obtained from endophytic fungus, EFB01 showed highest cytotoxicity. Endophytic fungus, EFB01 was selected for molecular identification. Genomic DNA was extracted using CTAB (Cetyl trimethyl ammonium bromide) buffer. The total genomic DNA concentration was estimated by absorbance at 260 nm. PCR standardization was carried out using ITS (Internal transcribed spacer) primers for endophytic fungus, EFB01.Using sequence analysis of ITS region and BLAST (Basic local alignment search tool) the endophytic fungus, EFB01 was identified as Colletotrichum gloeosporioides.

Keywords :Endophytic; Barringtonia acutangula; anticancer assay, Genomic DNA; PCR Standardization; BLAST of the endophytic fungus