International Journal of Current Microbiology and Applied Sciences
ISSN: 2319-7706 Volume 2 Number 2 (2013) pp.36 43

Isolation and Identification Vibrios from marine Seafood Samples

R.S. Gomathi, R.Vinothkumar and K. Arunagiri*
Department of Microbiology, Kanchi Shri Krishna College of Arts and Science, Kilambi,
Krishnapuram, Kancheepuram -631 551 Tamil Nadu, India.
*Corresponding author:


In this report encompasses the incidence of V. alginolyticus in sea foods as a human pathogen, in contribute to the onset of sporadic and epidemic outbreaks of diarrhoeal disease in humans. The following aspects were covered during the present investigation. Isolation of v. alginolyticus in sea food samples collected from Chennai, kanchipuram and Sriperumbudur local fish market. Antibiotic resistant pattern of isolated V.alginolyticus. Hemolytic activity of randomly selected in Vibrio alginolyticus. High lights of the pattern investigations are,V.alginolyticus was readily recoverable in all the 2 samples tested viz., Finfishes and crustaceans. All the biochemical test were carried out. Among 2 categories of samples, crustaceans harboured high number (80%) and finfishes (73%) of v.alginolyticus. Antibiotic resistant pattern was observed in Penicillin, Erythromycin, tetracycline, streptomycin, amoxyclav ampicillin, vancomyvin, and novobiocin. No resistant pattern was observed in Validixic acid, Gentamycin, Nitrofurantoin, Chloramphenical. A total of 15 strains were tested for hemolytic reaction 12 strains were positive for hemolytic production in blood agar plates and the remaining 3 strains were failed to lyses blood erythrocytes.

Keywords :Marine bacteria; Isolation of Vibrio; Marine fishes; Haemolytic activity; Antibiotic resistant pattern.