International Journal of Current Microbiology and Applied Sciences
ISSN: 2319-7692 Volume 2 Number 2 (2013) pp.15 19

Phytochemical and in vitro anti-diabetic activity of methanolic
extract of Psidium guajava leaves

R. Manikandan*, A.Vijaya Anand1 and G. Durai Muthumani2
*Department of Chemistry, SSK Polytechnic College, Poolangulathupatti,
Trichirappalli, Tamil Nadu, India.
1P.G. Department of Biochemistry, M.I.E.T. Arts and Science College,
Trichirappalli, Tamil Nadu, India
2Department of Biochemistry, Kanchi Shri Krishna College of Arts and Science, Kilambi,
Kancheepuram -631 551 Tamil Nadu, India.
*Corresponding author:


The intestinal digestive enzymes alpha-glucosidase and alpha-amylase are plays a vital role in the carbohydrate digestion. One antidiabetic therapeutic approach reduces the post prandial glucose level in blood by the inhibition of alpha-glucosidase and alpha-amylase enzymes. These can be an important strategy in management of blood glucose. The aim of the present study was to investigate the phytochemical bioactive compounds of the methanolic extract of Psidium guajava leaves, its in vitro anti-diabetic activity. The assay results suggests that the presence of bioactive compounds, could be responsible for the versatile medicinal properties of this plant including diabetes, the extract exhibit the dose-dependent increase in inhibitory effect on alpha-glucosidase enzyme (upto 89.4%), and alpha-amylase enzyme (upto 96.3%). The current study proves that the antidiabetic activity of methanolic extract of Psidium guajava leaves by in vitro studies.

Keywords :Psidium guajava; Phytochemical; alphaglucosidase; alpha-amylase