ABSTRACT

A total of 100 clinical samples were collected from different hospitals and microbiology laboratories in Kancheepuram district, Tamilnadu, India that includes Blood (15 samples), Pus swabs (25 samples), Stool (40), Urine (20 samples) for screening the E. faecalis. Among the 100 clinical samples 47 isolates showed Enterococcus faecalis. The bacterial isolates from the clinical specimens for the present study were confirmed as E. faecalis by using conventional tests. The antibiotic resistant of E. faecalis isolates showed a maximum of 53.1 percent to Penicillin-G which was followed by other antibiotics in the following order: kanamycin>Streptomycin> Ciprofloxacin>chloramphenical>amphicillin>Vancomycin. The leaves of three plant species Aegles maromoles, Aristolochia indica and Ocimum canum were collected and subjected to ethanolic extraction. The ethanolic leaves extracts were tested against clinically isolated Multidrug resistance Enterococcus faecalis and Standard strains of E. faecalis. Antibacterial reference standard, Vancomycin had equal effect on Multidrug resistant E. faecalis isolates from clinical isolates and Standard E. faecalis. Zone of inhibition of Vancomycin was compared with plant extracts. The antimicrobial activity of plant extract was higher in Hibiscus Sabdariffa than the other leaves extract tested against test bacteria. Zone of inhibition of the plant leaves extracts from Hibiscus Sabdariffa showed high antimicrobial activity against Multidrug resistant E. faecalis. There is a scope to use ethanolic extract of the leaves of Aristolochia indica against Enterococcal infections caused by Multidrug resistance E. faecalis.

Keywords :Antibacterial activity; Medicinal plants; multidrug resistant; Enterococcus faecalis; MDRE