International Journal of Current Microbiology and Applied Sciences
ISSN: 2319-7692 Volume 1 Number 1 (2012) pp.56–62

Isolation and characterization of silver nanoparticles from Fusarium oxysporum

1*K. Vanmathi Selvi and 2T. Sivakumar

1Department of Microbiology, Sri Akilandeswari Women’s College, Vandavasi,
T.V.Malai, Tamil Nadu, India.

2Department of Microbiology, Kanchi shri Krishna College of Arts & Science,
Kilambi, Kancheepuram, Tamilnadu, India- 631 551
*Corresponding author:



A total of 21 isolates of Fusarium sp. were isolated from soil samples collected from an area of mangrove forest in Pichavaram, Tamil nadu, India.  The isolates were isolated using soil dilution, direct isolation and debris isolation techniques. Based on identification using morphological characteristics, Fusarium oxysporum was identified. The extracellular production of silver nanoparticles by the fungus Fusarium oxysporum was investigated. It was found that exposure of Fusarium oxysporum to silver ion leads to the formation of silver nanoparticles. The silver nanoparticles were in the range of 5-60nm in dimension. The nanoparticles were examined using UV-Visible spectroscopy, scanning Electron Microscopy (SEM), X-ray Diffractometer (XRD), and Fourier Transform Infrared Spectroscopy (FTIR) analysis. The formation of nanoparticles by this method is extremely rapid, undertaken in ambient conditions and synthesized nanoparticles are stable for several months in the absence of light.

Keywords : Mangrove soil; Fusarium oxysporum; Silver nanoparticles; Chacterization of nano particles