International Journal of Current Microbiology and Applied Sciences
ISSN: 2319-7692 Volume 1 Number 1 (2012) pp.40–49.


Partial purification and characterization of Anti- MRSA Peptide from Marine Pseudomonas aeruginosa

                       1*V.Shanmugaraju, 1R.Chidambararajan and 2N.Sivakumar

1Department of Biotechnology, Dr.N.G.P. College of Arts & Science
Dr NGP Nagar, Coimbatore, Tamil Nadu, India - 641 048.

2Dept.of Biology, Sultan Qaboos University, Muscat, Oman.
*Corresponding author:


Antimicrobial peptides are so widespread that they are likely to play an important protective role. Marine bacterium has been recognized as important antimicrobial peptide producers which have an exceedingly bright future in the discovery of life saving drugs. Wet soil samples were collected from marine resource for the isolation of bacteria. 14 bacteria’s were isolated based on their morphological and structural characteristics. They were screened for antibacterial activity against MRSA by point inoculation and agar well diffusion method. Screening for antibacterial activity of the supernatant of A was also done. In all the screening processes isolate A showed zone of clearance of 12mm. The marine isolate A was identified by 16S rDNA gene sequencing as Pseudomonas aeruginosa. Antimicrobial substance produced by Pseudomonas aeruginosa was partially characterized. The protein was isolated using ammonium sulphate precipitation method and desalted via desalting column. The molecular weight of the protein determined SDS PAGE was 30kDa. MALDI – TOF MS of the peptide solution revealed four different fractions of the peptide which were in the molecular mass 5427.9, 3162, 5818.7, 1421 Da. Scanning electron microscopic analysis revealed the topographic changes in the cell. From the present study it has been concluded that the peptide from Pseudomonas aeruginosa has greater antibacterial activity against MRSA than Neomycin and Streptomycin. The findings of this surveillance study will enhance our knowledge regarding the problem of antimicrobial resistance and will serve as a basis for future development of new anti – MRSA substance against new resistance in MRSA.

Keywords : Antimicrobial peptide; Electron Microscope Studies; 16s RNA studies; Characterization of antimicrobial peptide