International Journal of Current Microbiology and Applied Sciences |
ISSN: 2319-7706 Volume 2 Number 9 (2013) pp. 188-197 |
Biocatalyst activity of entomogenous fungi: stereoselective reduction of carbonyl compounds using tochukaso and related species |
K. Ishihara1*, H. Fujimoto1
, M. Kodani1
, K. Mouri1
, T. Yamamoto1
, M.Ishida1
, K. Maruike1
, H. Hamada1
, N. Nakajima2
and N. Masuoka |
1Department of Life Science, Okayama University of Science, Okayama, Japan
2Department of Nutritional Science, Okayama Prefectural University, Soja, Okayama, Japan
*Corresponding author e-mail: ishihara@dls.ous.ac.jp |
ABSTRACT |
To investigate the potential ability of tochukaso and related species to act as biocatalysts, we screened 13 entomogenous fungal strains. Two recommended media (potato-dextrose broth and 889 media) and a modified medium (PGO medium) were tested for liquid culture of these entomogenous fungi. Six strains
(NBRC100741, 100742, 101754, 106941, 106945, and 106950) cultured using the PGO medium showed a good growth. The stereoselective reduction of -keto esters and aromatic -keto amide using these six strains was investigated. It was
found that these strains possess a reducing activity toward various -keto esters. Among them, the reduction of -keto esters by Isaria takamizusanesis NBRC100741 in the presence of L -glycine as an additive gave the corresponding
-hydroxy esters with a high conversion ratio and excellent enantioselectivity. Furthermore, it was found that the NBRC100742 strain reduced 2- chlorobenzoylforamide to (R)-2-chloromandelamide with excellent enantiomeric
excess even at a high substrate concentration (75.0 mM). Thus, we found that
tochukaso and related species have great potential to be used as biocatalysts for the
stereoselective reduction of carbonyl compounds. |
Keywords |
Tohchukaso and related species; Entomogenous fungi; Biocatalyst; Stereoselective reduction; Chiral alcohol. |