International Journal of Current Microbiology and Applied Sciences |
ISSN: 2319-7706 Volume 2 Number 9 (2013) pp. 160-163 |
Comparative assessment on antibacterial activity of bark of Carallia brachiata (Lour.) Merr prepared in single and gradient extraction methods |
Anju Abraham and Toji Thomas* |
Post Graduate and Research Department of Botany, St. Thomas College Palai, Arunapuram P.O. Pala, Kerala-686574, India *Corresponding author e-mail: tojidr@yahoo.com |
ABSTRACT |
Bark of Carallia brachiata was evaluated for antibacterial activity in gradient extraction and single extraction (95% ethanol) methods. Gradient extraction was conducted using petroleum ether, acetone, ethanol, and water as extracting solvents
in the gradation of increasing polarity. Single extraction method is common in
homeopathic medicine preparations. We compared antibacterial activity in both methods. Antibacterial activity was tested towards ten pathogenic bacterial strains. The antibacterial activity was observed at its maximum in acetone extract prepared
in gradient fashion. All the 10 bacterial strains showed considerable inhibition of growth in acetone extract. Acetone extract of the bark showed like minimum
inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values of 12.5mg/ml and 25mg/ml. towards Serratia marcescens. Single ethanolic
extraction method is commonly employed for preparing medicines in Homeopathy; our investigation showed that gradient extraction method could give better results compared to single extraction in 95% ethanol. |
Keywords |
Carallia brachiata; antibacterial activity;
bark extracts; gradient extraction; disc diffusion. |