International Journal of Current Microbiology and Applied Sciences
ISSN: 2319-7706 Volume 2 Number 8 (2013) pp. 347-358
Isolation of bacteria from seacoasts and screening for its antibacterial substance production
V. Shanmugaraju1 , P. Arun1 , J. Rengaramanujam2 and S. Senthilprabhu2
1Department of Biotechnology, Dr.NGP Arts and Science College, Coimbatore-48, Tamilnadu, India 2Department of Microbiology, Dr.NGP Arts and Science College, Coimbatore-48, Tamilnadu, India *Corresponding author e-mail: rajugenes@yahoo.co.in
A B S T R A C T
Ten marine bacteria were isolated and isolated for its antibacterial substance production against ten clinical isolates. The screening was carried out by different methods such as point-inoculation method, Agar-well diffusion method and screening of supernatant for antibacterial substance production. After point inoculation method and agar-well diffusion method, inhibitory activity against atleast one clinical isolate was detected for six marine isolates. The marine isolate M5 with more antibacterial activity was identified as Lysinibacillus sphaericus by 16S rRNA gene sequencing
Keywords
Marine bacteria; Antibacterial substance; Pathogens.