International Journal of Current Microbiology and Applied Sciences

ISSN: 2319-7706 Volume 2 Number 8 (2013) pp. 102-109

Molecular Identification of Aedes albopictus (Diptera: Culicidae) and Quantitative Analysis of COI gene in South-Indian species.

Sambashiva Daravath1 , M. Madhavi Yadav 2 , P.Chakrapani2 and B.Reddya Naik1 *

1Department of Zoology, Osmania University, Hyderabad, India- 500007 2Department of Genetics, Osmania University, Hyderabad, India- 500007 *Corresponding author e-mail: shivabt07@gmail.com

A B S T R A C T

The alarming fact is that the dengue fever has become endemic in the urban areas of India and especially in Hyderabad; there are about 200 confirmed and 181 suspected dengue cases were reported in the year 2012. In our study we found that Aedes albopictus is a major vector for dengue in Hyderabad. Rapid, accurate and timely identification of mosquitoes has become a challenging task, especially for mosquito control programme managers. The CO I gene of mitochondria is used as powerful taxon gene in molecular identification of the species. DNA barcoding is a method for the identification of species in a wide range of animal taxa, which uses the 5 region of the mitochondrial cyto chrome C oxidase-I (CO-I) gene. The Aedes albopictus mosquito has 600 bp length of CO I gene and shows distinct variation when compared with the other species. The CO I gene is amplified and sequenced in this study. The data has been submitted to NCBI gene bank and shown 100% similarity of COI gene with that of different geo graphically located Aedes albopictus species. The Hyderabad region species shows significant variation at amino acid percentage within Indian population of Aedes albopictus.

Keywords

Dengue; Aedes albopictus; CO I gene; Amino acids; Mosquito.