International Journal of Current Microbiology and Applied Sciences

ISSN: 2319-7706 Volume 2 Number 7 (2013) pp. 63-68

Comparative study of invasive methods for diagnosis of Helicobacter pylori in humans

V.Subbukesavaraja1 * and K.Balan 2

1Department of Microbiology, MES Medical College, Kerala, India 2Department of Microbiology, KIMS, Tamil Nadu, India *Corresponding author e-mail: arsenio82@gmail.com

A B S T R A C T

To compare the various invasive tests (Rapid urease, conventional Urease, Gram stain, Geimsa stain) for identification of Helicobacter pylori. (H.pylori). A total of 81 endoscopic biopsy specimens were collected from gastric antrum of both the sexes in age group 20-70 over a period of 12 months from tertiary care hospital. Of 81 biopsy specimen, 59 were male (72.4%) and 22(27.6%) female, and the maximum number of patients was in the age group 40-49. The endoscopy results revealed that duodenal ulcer accounted for 36%, gastritis in 30%, gastric carcinoma in 16%, and gastric ulcer in 17%. In the present study rapid urease detected 35 positive cases, Giemsa staining 22 cases and Grams stain detects 18 cases. Majority of cases were in the age group of 40-49 years of male preponderance. Rapid urease test was positive in 43.21% of the samples of which 64.15% were positive in the first 10 minutes and Gram stain was positive in 22.2% whereas Giemsa stain was positive in 27.1%. More number of samples was found to be positive for H.pylori by Giemsa stain (27.1%) as compared to those prepared by Gram stain (22.2%).

Keywords

Biopsy; urease test; Gram s stain; Giemsa stain; Helicobacter pylori.