International Journal of Current Microbiology and Applied Sciences
ISSN: 2319-7706 Volume 2 Number 7 (2013) pp. 171-187
Dynamics of Microorganisms during Vermistabilization of organic substrates and enhances performance of Plant Growth Promoting Rhizobacteria on Black Gram
M.Prakash*, and N. Hemalatha
Department of Microbiology, Kanchi Shri Krishna College of arts and Science, Kilambi, Kancheepuram-631 551, Tamilnadu, India *Corresponding author e-mail: mprakashmicro@gmail.com
A B S T R A C T
The Quality of the, Paper mill sludge, Leaf litter and Press mud before and after 60 days of vermicomposting was investigated on Physico-chemical and microbiological methods. Vermistabilization of Paper mill sludge, Leaf litter and Press mud sludge by using Epigeic species of Eisenia fetida in three different vermibed substrates by using cow dung as the supporting material. The bulk density on 0th day in Paper mill sludge, Leaf litter and Press mud with cow dung incorporated sludge sample were higher than the control and compost obtained on 60th. The chemical analysis of N, P and K also showed increasing trend on 60th day composting than the initial 0 th day and control sample in all the three organic substrates. The total bacterial count on the 60th day of composting in three vermibed substrates with cow dung incorporated Paper mill sludge; Leaf litter and Press mud were tremendously higher than the 0th day and control sample under investigation. The principal bacterial genera encountered in Paper mill sludge, Leaf litter and Press mud on the initial 0th day and 60 th day comprised of 4 bacterial sps includes Bacillus, Pseudomonas, Rhizobium, and Azotobacter. Few PGPR tolerant to multiple heavy metals and exhibiting a couple of resistant activities mainly Pseudomonas sps were resistant to 400 gml-1 of Hg. It was apparent that cultures of PGPR isolated from vermicompost were tolerant to elevated levels heavy metals. The plant growth parameters Shoot length, root length, number of roots hairs, Number of root nodules and number of branches and leaves were determined and also yield of Black gram (Vigna mungo L. Hepper) was determined for selecting the right organic input and plant growth promoting rhizobacteria.
Keywords
Paper mill sludge; Leaf litter; Press mud Bacillus, Pseudomonas, Rhizobium; Azotobacter; PGPR; Vigna mungo L. Hepper; Vermi- stabilization