International Journal of Current Microbiology and Applied Sciences |
ISSN: 2319-7706 Volume 2 Number 7 (2013) pp. 171-187 |
Dynamics of Microorganisms during Vermistabilization of organic substrates and enhances performance of Plant Growth Promoting Rhizobacteria on Black Gram |
M.Prakash*, and N. Hemalatha |
Department of Microbiology, Kanchi Shri Krishna College of arts and Science, Kilambi, Kancheepuram-631 551, Tamilnadu, India *Corresponding author e-mail: mprakashmicro@gmail.com |
A B S T R A C T |
The Quality of the, Paper mill sludge, Leaf litter and Press mud before and after 60
days of vermicomposting was investigated on Physico-chemical and microbiological methods. Vermistabilization of Paper mill sludge, Leaf litter and Press mud sludge by using Epigeic species of Eisenia fetida in three different vermibed substrates by using cow dung as the supporting material. The bulk
density on 0th day in Paper mill sludge, Leaf litter and Press mud with cow dung
incorporated sludge sample were higher than the control and compost obtained on
60th. The chemical analysis of N, P and K also showed increasing trend on 60th day composting than the initial 0
th day and control sample in all the three organic
substrates. The total bacterial count on the 60th day of composting in three vermibed substrates with cow dung incorporated Paper mill sludge; Leaf litter and Press mud were tremendously higher than the 0th day and control sample under
investigation. The principal bacterial genera encountered in Paper mill sludge, Leaf
litter and Press mud on the initial 0th day and 60
th day comprised of 4 bacterial sps
includes Bacillus, Pseudomonas, Rhizobium, and Azotobacter. Few PGPR tolerant
to multiple heavy metals and exhibiting a couple of resistant activities mainly Pseudomonas sps were resistant to 400 gml-1
of Hg. It was apparent that cultures of PGPR isolated from vermicompost were tolerant to elevated levels heavy metals. The plant growth parameters Shoot length, root length, number of roots hairs, Number of root nodules and number of branches and leaves were determined and
also yield of Black gram (Vigna mungo L. Hepper) was determined for selecting
the right organic input and plant growth promoting rhizobacteria. |
Keywords |
Paper mill sludge; Leaf litter; Press mud Bacillus, Pseudomonas, Rhizobium; Azotobacter; PGPR; Vigna mungo L. Hepper; Vermi- stabilization |