International Journal of Current Microbiology and Applied Sciences

ISSN: 2319-7706 Volume 2 Number 10 (2013) pp. 467-476

Optimization of culture condition for enhanced decolorization of Reactive Orange 16 by Comamonas acidovorans MTCC 3364

Darshan M. Rudakiya* and Kirti S. Pawar

Ashok & Rita Patel Institute of Integrated Study and Research in Biotechnology and Allied Sciences, New VallabhVidyanagar, Anand, Gujarat

ABSTRACT

Many synthetic azo dyes and their metabolites are toxic, carcinogenic, and mutagenic so removal of azo dyes using cost-effective and eco-friendly method is major aspect.Comamonas acidovorans MTCC 3364 has been routinely reported for different steroid bioconversion and heavy metal removal. The main purpose of this study is to check the decolorization efficiency of Comamonas acidovorans MTCC 3364 for different dyes and to optimize the condition which gives maximum decolorization of Reactive Orange 16 dye. The effect of various physicochemical parameters including condition, carbon and nitrogen sources, temperature,pH and dye concentration were studied. The % decolorization of dye was determined by UV Visible spectroscopy. This bacterial strain efficiently decolorizes Reactive Orange 16 at 37 oC, pH 6.85 within 24 hours giving 99.03 ± 0.5 % dye decolorization under optimum environmental conditions.

Keywords

Comamonas acidovorans MTCC 3364; Reactive Orange 16; Bioremediation; Azo dye decolorization.