International Journal of Current Microbiology and Applied Sciences

ISSN: 2319-7706 Volume 2 Number 10 (2013) pp. 172-178

Detection of Treponema denticola by PCR in patients with different periodontal status

Varsha Chaudhary1 , Bhat Kishore1 , Sunil Rao2 , Manohar Kugaji1 and Preeti Ingalagi1

1Department of Microbiology, Maratha Mandal s NGH Institute of Dental Sciences and Research Centre, Belgaum, India 2Department of Microbiology, Yenepoya Medical College, Yenepoya University, Mangalore, India

ABSTRACT

The aim of the study was to determine the prevalence of T.denticola by 16S rRNA- based PCR technique in subgingival plaque of periodontally diseased and healthy subjects and its relationship with the periodontal status. T. denticola is a highly motile, aerotolerent and anaerobic spirochete. It has been predominantly associated with the incidence and severity of human periodontal disease. As it is difficult to isolate and identify T.denticola from clinical plaque samples, currently, 16S rRNA- based PCR method continues to be one of the most sensitive , rapid, and cost- effective methods for determining the prevalence of such microorganisms. A total of three hundred subjects divided into three catogories namely; Chronic periodontitis (CP=100), Aggressive periodontitis (AP=100) and controls (C=100) were selected for the study in an age range of 20 to 50 years. Subgingival plaque samples were collected and forwarded for PCR analysis. The PCR was performed by using specific primers for the 16S rRNA gene of T.denticola and quantification was done using standard strain of T.denticola. Detection frequencies of T.denticola in plaque samples from AP patients and CP patients were reported much higher than those from healthy subjects. There was a significant difference between the PCR detections of CP and Control groups. Results of PCR depicted that Female were prone to chronic periodontitis than male in all age groups. This study demonstrated the utility of a 16S rRNA-based PCR detection method for identifying T.denticola and strong association between T.denticola and periodontitis.

Keywords

Aggressive periodontitis; Chronic periodontitis; PCR; Treponema denticola.