National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The main objective is to evaluate the incidence of bacterial pathogens in the liquid bile of patients underwent laparoscopic cholecystectomy using CHROMagar media. A Total of 34 samples were collected from patients suffering from cholecystolithiasis who were subjected to laparoscopic cholecystectomy by the same surgeon at Al-Sadr Teaching Hospital in Basra City for the period (31st of January 2016 to 30th of April 2016). It included 32 (94.1%) females and 2 (5.9%) males, aging between (23 – 61 years). Bile aspirates taken from each gallbladder in the operative room and directly inoculated onto brain heart infusion growth and incubated at 37°C for (24-48 hours). The isolates were identified by routine biochemical tests and CHROMagar media. A total of 34 samples were examined for detection of bacteria showed E. coil 11(32.4%), Enterococcus spp 7(20.6%), Pseudomonas aeruginosa 4(11.8%), Klebsiella pneumoniae 3(8.8%), Salmonella spp 3(8.8%) and Staphylococcus aureus 2(5.9%). A screening procedure using CHROMagar media gives better results and faster in identifying bacteria than conventional methods. Still the stasis secondary to gall stones is the commonest cause for cholecystitis and bacterial infection. Our work wishes to emphasize that E. coli presents at high frequency among other bacteria.
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