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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 5, Issue:9, September, 2016

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com /
submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(9): 843-849
DOI: http://dx.doi.org/10.20546/ijcmas.2016.509.095


An Investigation of RAPD Analysis of Genetic Variability of Some Rice Germplasm (Oryza sativa L.)
B.R. Roopa and B.K. Chikkaswamy*
Department of Biotechnology, Sigma Bioscience Research center, # 530, 16th Cross, 2nd Stage, Indiranagar, Bangaluru-560038, India
*Corresponding author
Abstract:

The number of polymorphic fragments for each primer varied from 1to3 with an average of 2.74 polymorphic fragments. The primer OPA-18 produced the maximum number of polymorphic bands. The RAPD data was analyzed to determine the genetic similarity coefficients which ranged from 0.46 to 0.91. Cluster analysis was performed using Ward’s method) using the Jaccard’s similarity coefficient. The Wards method dendrogram resolved the selected rice cultivars into two to three major clusters. The genetic diversity in 27 selected cultivars of rice, Oryza sativa L. using 30 decamer random primers. Out of 30, 25 RAPD primers revealed polymorphism while the remaining 5 primers showed no reaction. The primers produced a total of 74 bands of which 69 were polymorphic (96.2%).


Keywords: Genetic variation molecular Markers, Oryza sativa Ward’s method.

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How to cite this article:

Roopa, B.R.,  and Chikkaswamy, B.K. 2016. An Investigation of RAPD Analysis of Genetic Variability of  Some Rice Germplasm (Oryza sativa L.).Int.J.Curr.Microbiol.App.Sci. 5(9): 843-849. doi: http://dx.doi.org/10.20546/ijcmas.2016.509.095
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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