National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
A bacterial isolate (I5) along with Bacillus subtilis and Bacillus circulans was evaluated for extracellular amylase production under SmF and SSF conditions. In SmF (Temperature - 28±2°C, pH -7 and inoculum size-106 cfu ml-1, Time- 96 h), maximum saccharifying activity (SA) was observed with Bacillus subtilis (0.62 IU/ml/min) followed by isolate (I5) (0.58IU/ml/min) and Bacillus circulans (0.46IU/ml/min). Whereas dextrinizing activity (DA) was recorded with Bacillus circulans (1.69U/ml), followed by isolate (I5) (1.65U/ml) and Bacillus subtilis (1.02U/ml). In SSF(1:2 moisture content, incubation time -96 hours, pH-7inoculum size-106 cfu ml-1 Temp-37°C) using different substrates (potato starch, corn starch, raw wheat flour and rice starch) as carbon sources. Maximum saccharifying activity (23.10IU/ml/min) was expressed by Bacillus circulans in 1% rice starch at 28±2°C. Whereas highest dextrinizing activity was recorded with 1% corn starch (3.31U/ml). On the basis of the present study, Bacillus circulans being the most potential strain for maximum and cost effective alpha amylase enzyme production at appropriate conditions could be utilized as bio-inoculant in fermentation technology in sustainable manner.
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