National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
A comprehensive study was performed to examine the incidence of different Aeromonas species, their drug sensitivity patterns and virulence genes isolated from diarrhoea patients and environmental water samples between September, 2006 to August, 2007 in Odisha. The rectal swab and water samples were bacteriologically analyzed using standard techniques; detection of toxic and clonality were done by PCR and RAPD-PCR Assay respectively. Out of 280 diarrhoea samples and 100 water samples, 20.2% from diarrhoea patients and 62.5% from water samples were positive for Aeromonas spp. A. hydrophila (50.9%) was isolated as dominant pathogen followed by A. caviae (27.2%) and A. sobria (21.8%). The common resistance profile of three different Aeromonas species were piperacillin, kanamycin, bacitracin, rifampicin, erythromycin, sulfadiazine, roxithromycin, lomefloxacin, ciprofloxacin, ampicillin, furazolidone, cefuroxime, and amoxycillin. On blood agar plates 52.7% of Aeromonas spp. showed β- hemolysis and production of hly A gene as revealed by PCR assay. RAPD PCR assay revealed that the Aeromonas spp. were quite similar in clonality. As diarrhoea is the most promising health problem in Odisha, this type of study should be extended for longer period in different parts of the state.
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