National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The prevalence of human pathogenic bacteria especially Salmonella species in cow dung as a selected environmental sample was investigated. Five different abattoirs in Anambra State, Nigeria were used. Potentially pathogenic organisms were isolated and identified and they include; Salmonella enteritidis, Shigella sp., Clostridium perfringens, Pseudomonas sp., E. coli, Staphylococcus aureus, Vibrio cholerae and Vibrio parahemolyticus. The susceptibility of the isolates to different antibiotics was tested and it was observed that Pseudomonas sp. was very sensitive to the antibiotics, Ceporex (10µg) and Tarivd (10µg) with 16mm zone of inhibition. Clostridium sp. was more susceptible to Levofloxacin (20µg) with 20mm zone of inhibition, Staph. aureus was more susceptible to Streptomycin (30µg) and Levoflaxacin (20µg) with 18mm zone of inhibition, E. coli was more susceptible to Gentamycin (10µg) with 20mm zone of inhibition, Salmonella enteritidis was more sensitive to Augmentin (30µg) with 20mm zone of inhibition and Shigella sp. was more sensitive to Ciproflox (10µg) with 20mm zone of inhibition. The pathogenicity of these isolates was studied by infecting each on mice. There was death of two mice infected with Clostridium perfringens .Mice infected with Shigella sp., suffered swollen of the scrotum and scrotal sac which was observed after dissection. Mice infected with Pseudomonas sp., Staphylococcus aureus, and Salmonella enteritidis and E. coli, 25x108, 8x108, 20x108, 10x108 cfu/ml of the infected organisms were recovered from their intestine respectively. This shows that the organisms colonized their intestine at high level and they shed them in their faeces, though the infections were asymptomatic at the stage.
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