National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Nitrogen is an essential macromolecule necessary for suitable growth and development of plants by playing a vital role in biochemical and physiological functions throughout the life cycle. Early node 93 (ENOD93-1) gene is well known for significantly responding to both N induction and N reduction in plants, but little is known about the structure and function of the ENOD93-1 protein in cereal crops. In this study, a putative ortholog of rice OsENOD93-1 gene was identified in the wheat genome. BLASTP analysis showed the OsENOD93-1 shared the 80% similarity with wheat TRIAE_CS42_4AL_TGACv1_ 288261_AA0942630.1 scaffold. Present study describes the location of TaENOD93-1 gene on4AL chromosome of wheat. MicroRNA target analysis revealed that TaENOD93-1 gene targeting by miR6201. Phylogeny tree suggested that identified putative ortholog shared the evolutionary relationship with monocots. The three dimensional structure of TaENOD93-1 protein was predicted using computer-aided molecular modeling technique followed by structure evaluation using Ramachandran plot. The primary goal of this study was to mapping the chromosomal location and functional annotation of TaENOD93-1 gene in wheat using bioinformatics approach. The identification of putative ortholog of OsENOD93-1 gene in wheat has opened up new opportunities to improve the nitrogen use efficiency (NUE) in wheat through molecular breeding approach.
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