National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Extracellular lipase production by Streptomyces fungicidicus RPBS-A4, previously isolated from the Bay of Bengal has been investigated in submerged fermentation using different proportions of disposed marine wastes such as fish, shrimp and crab as nitrogen source. Among the tested, shrimp waste supported the maximum lipase production. The effect of carbon sources on lipase production revealed that lactose aided the higher lipase production than any other tested carbon source and a concentration of 1.5% lactose registered as optimum to enhance the lipase production. Among the nitrogen sources tested, lipase production was high in peptone added medium when compared to other nitrogen sources, and its optimum concentration recorded was 1.5%. Partial characterization of crude enzyme revealed that pH 9.0 and 45°C temperature were optimum for maximum lipase activity.
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