National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Helicobacter pylori infection is a factor in the pathogenesis of gastroduodenal mucosal inflammation and gastric carcinoma. Though Histopathology plays a pivotal role in diagnosis of H.pylori infection, it has several limitations. This study was aimed at evaluating an in-house urease test and crush cytology for diagnosis of H. pylori infection. This cross sectional study was done for a period of three months on outpatients undergoing upper gastrointestinal endoscopy for various clinical indications. Antral biopsy specimens were obtained from 120 patients with findings of gastroduodenitis with or without gastric and duodenal ulcer. The samples were subjected to the following tests namely an in-house rapid urease test (RUT), Giemsa staining of crush cytology and histopathology. Kappa statistics was used to evaluate the various tests. RUT was positive in 45% of patients, Giemsa staining of crush cytology in 44.2% and histopathology in 45.8%. Considering histopathology report as the gold standard. Kappa statistics showed almost perfect degree agreement of HPE with Giemsa staining of crush cytology (Kappa-0.96 and 98% agreement) and RUT (Kappa 0.92 and 96% agreement). The sensitivity and specificity of Giemsa stain was 96.4% and 100% respectively and that of RUT was 94.5% and 96.9% respectively. Giemsa stain of crush cytology is a rapid and specific diagnostic test and is comparable to histopathology for the diagnosis of H. pylori infection.
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