National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The present study was taken up with a view to undertake plaque purification of BTV-4 isolate available in the department, to confirm its purity. BTV-4 isolate was adapted to Vero cell line and had a titer of 106.46/ml after 8 passages in the process of plaque purification which was determined as 104.833/ml prior to adaption. Plaque purification was carried for three times using agarose overlaying method in 6 well plate. Each time plaques were observed from 4th day of infection of approximately not more than 1mm diameter. One of the plaques from third round of purification was grown in T25 flask and the resultant cell culture fluid was used for RNA isolation and RT-PCR through which the respective plaque was confirmed as BTV-4. Therefore, it was confirmed that there was no other serotype other than BTV-4 and the plaque purified virus can be used either to study pathogenesis or to raise hyperimmune serum.
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