National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Banana and plantains is the most important tropical fruit crop in the world, which serves as a major food and source of income in many countries. Bananas are severely infected by several pathogens one such Fusarium oxysoporum f. sp. Cubense which causes significant crop losses in the fields. In this study fusarium wilt screening was carried out on chemically induced mutants and molecular analysis was done to assess the acquired resistance to Fusarium oxysoporum f. sp. cubense. From the early screening we observed that Ethyl Methane Sulphonate derived mutants are more sensitive which ranged from 9.5-11.4%. Whereas, several mutants obtained from Sodium Azide were resistant and was comparable with resistant cv. Poovan. Similar correlation analysis with molecular markers also revealed comparable results with the early screening. RAPD primers (OPN-06 and OPR-07) showed resistance specific bands and were used for the testing variation among mutants, after confirming its reproducibility. Putative resistant and susceptible mutants along with Poovan banana when amplified with OPN 06 showed the presence of a major band at 1500 bp and 200 bp which evidenced the occurrence of random mutations in genome.
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