National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The effects of different doses (0, 10, 30, 50 and 60 Gy) of radioactive cobalt (60Co) rays on axillary buds of strawberry were investigated under in vitro conditions. The results showed that irradiated buds had increased shoot proliferation percentage, average number of shoots, shoot length, average number of roots, root length, root initiation percentage, survival percentage and number of leaves. However, at higher doses it showed inhibitory effects and significant decreases in all above parameters was observed. The highest shoot proliferation per cent (91.06 %), average number of shoots (8.44) and shoot length (2.77 cm) was recorded when buds were irradiated with 10 Gy gamma dose followed by 30 Gy gamma dose. Gamma doses over 30 Gy resulted in sharp decreases in all parameters examined. The highest root initiation percentage (91.06 %), average number of roots (5.58) and root lengths (3.62 cm) was recorded from 10 Gy gamma rays. After 7 weeks of hardening maximum survival per cent (86.66 %) was recorded in un-irradiated (control) buds followed by buds irradiated with 10 Gy dose whereas, maximum number of leaves were recorded in case of cultures irradiated with 10 Gy. Thus it can be concluded that lower doses of gamma rays can improve the survival and growth of explants under in vitro conditions.
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