National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Fungi are microorganisms considered decomposers of the organic matter, by the action of several enzymes that can present biotechnological potential, emphasizing the holocelulases (endoglucanases, exoglucanases, β-glucosidases, endoxilanases, β-xylosidases, among others). Among holocellulolytic fungi described in literature, Penicillium, Aspergillus and Trichoderma species are the most important. This study aimed to evaluate the efficiency in production of holocellulolytic accessories enzymes (ï¢-glucosidase, ï¢-xylosidase and ï¡-L-arabinofuranosidase) by Trichoderma atroviride 102C1, a mutant strain obtained in our laboratory, using different lignocelluloses biomass as substrates. The mutant strain 102C1 was cultivated at 28oC in salt mineral solution supplemented with corn steep liquor as nitrogen source (1.26% w/v), and wheat bran, sorghum bagasse, sugarcane straw (in natura and pre-treated with steam explosion) or sugarcane bagasse (in natura and pre-treated with steam explosion), as carbon source (2.5% w/v), 200 rpm, for 5 days. The best results for (ï¢-glucosidase, ï¢-xylosidase and ï¡-L-arabinofuranosidase activities were observed in sorghum bagasse (13.09 U.ml-1), sugarcane straw pretreated (5.24 U.ml-1) and wheat bran (192.6 U.ml-1), respectively, between 3 and 5 days fermentation. These results suggest the use of different agro-industrial by products to obtain holocellulolytic accessories enzymes by the mutant fungi T. atroviride 102C1 and the possibility for biotechnology process.
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