National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
In this study systematic bacteriological examination of aseptically collected 150 shrimp samples, 27 samples of hand swabs from fish handlers were collected from retail fish outlets, and 23 stool samples from patients suffered with digestive disturbances after consumption of seafood reported to private clinics, all from Navsari city were investigated. The samples were enriched in Alkaline Peptone Water followed by direct plating on selective media viz. Thiosulfate Citrate Bile Salt Sucrose agar and Vibrio parahaemolyticus sucrose agar. All samples were brought to the laboratory for isolation, identification and characterization of V. parahaemolyticus isolates on the basis of their morphological, cultural and biochemical characteristics. Out of total 150 samples of shrimp 5 (3.33%) isolates of V. parahaemolyticus were obtained which included 3 (4.28%, 3/70) from marine shrimp and 2 (2.5%, 2/80) from freshwater shrimp samples. Out of 27 samples of the hand swabs of fish handlers, 2 (7.40 %) were positive for V. parahaemolyticus. However, none of 23 human stool samples was positive for the pathogen. All the 7 V. parahaemolyticus isolates were further processed to study drug resistance pattern against 7 commonly used antibiotics by agar disc diffusion method. The V. parahaemolyticus isolates showed cent percent sensitivity towards Ciprofloxacin and Gentamicin, followed in descending order by Amikacin (85.71%), Trimethoprim (85.71%), Streptomycin (71.42%) and Tetracycline (28.58%). An intermediate sensitivity of isolates was noticed against Streptomycin (28.58%) and Amikacin (14.29%). All V. parahaemolyticus isolates were totally resistant to Penicillin. However, varying degree of resistance was observed against Tetracycline (71.42%) and Trimethoprim (14.29%).
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