National Academy of Agricultural Sciences (NAAS)
|
PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Biofilms are group of microorganisms encased in an exopolymeric coat. They have been associated with a variety of persistent infections that respond poorly to conventional antibiotics. To evaluate three different methods for detection of biofilm formation in staphylococci. For detection of biofilm formation, 120 clinical isolates of staphylococcus spp. were screened by Congo red agar (CRA) methods, Tube methods (TM) and Tissue culture plate (TCP) methods. Out of 120 Staphylococcus spp., 72 were coagulase positive staphylococci (CPS) and 48 were coagulase negative staphylococci (CNS). 59.72% of CPS and 58.33% of CNS were slime producers. 71 isolates were detected as slime producer by TCP method, 56 by TM and 34 by CRA method. High resistances to conventional antibiotics were shown by biofilm producers. The TCP method was found to be most sensitive, accurate and reproducible screening method for detection of biofilm formation by staphylococci and has advantage of being a quantitative model to study the adherence of staphylococci on bio-medical devices.
Citations |
 |
 |
 |
 |
