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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 5, Issue:7, July, 2016

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com /
submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(7): 289-294
DOI: http://dx.doi.org/10.20546/ijcmas.2016.507.030


A Comparative Analysis of Enzyme-Linked Immunosorbent Assay and Rapid Card Test for Diagnosis of Rotavirus Antigen in Acute Diarrhea Below Five Years Children
Jugal Kishor Agarwal1*, S.P. Garg1, Dayachand2 and Dipty Agrawal3  
1Department of Microbiology, SIMS, Hapur, India
2Department of Pediatrics, SIMS, Hapur, India
 3Department of Anesthesia, SDN Hospital, Delhi, India
*Corresponding author
Abstract:

Group-A Rotavirus are responsible for severe watery diarrhea in young children. Rapid diagnosis of Rotavirus associated diarrhea can prevent inappropriate administration of antibiotics and help in preventing the spread of multi- drug resistance.  Rotavirus antigen is detected by ELISA and with one step rapid antigen test showed an incidence of 22% using ELISA and 21.33% using Rapid test respectively. Rapid test showed a sensitivity of 96.97% and specificity of 100% in comparison to ELISA. The Rotavirus infection was highest in age group of 6 months to 24 months (62.77%) and in male (63.64%). The infection was maximum during winter and presented with triad of diarrhea, vomiting and fever. Majority of cases had watery diarrhea with severe dehydration.


Keywords: ELISA,Group A Rotavirus, Immuno-chromatography, Watery diarrhea.

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How to cite this article:

Jugal Kishor Agarwal, S.P. Garg, Dayachand and Dipty Agrawal. 2016.  A Comparative Analysis of Enzyme-Linked Immunosorbent Assay and Rapid Card Test for Diagnosis of Rotavirus Antigen in Acute Diarrhea Below Five Years ChildrenInt.J.Curr.Microbiol.App.Sci. 5(7): 289-294. doi: http://dx.doi.org/10.20546/ijcmas.2016.507.030
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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