National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Fluorescence microscopy allows an extensive variety of analyzes in structural biology. Until the beginning of the seventeenth century living beings were limited to macroscopic organisms only, and there was no knowledge of the microscopic universe. Epi-fluorescence and Confocal microscopy appears in 1995 by Marvin Minsky with the aim of increasing the contrast and constructing three-dimensional images by the use of an aperture, allowing a great definition. The use of this microscopic technique is indicated for studies of thin or thick materials, live or pre-fixed and with or without staining by fluorophore molecules. In this study we present the applicability of fluorescence microscopy to identify damage in the cytoplasmic membrane of prokaryotes and mechanisms of cell death in eukaryotes.
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