National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Fourteen isolates were obtained from poultry and beam house region soils of Sangli district. An isolate showing maximum keratinolysis was identified as Streptomyces coelicoflavus. The ability of Streptomyces coelicoflavus to utilize chicken feather as a substrate was tested. Crude extract of keratinase enzyme from feather meal broth was used for characterization. It was found that maximum enzyme activitywas49U/ml. Crude keratinase was optimally active in the pH range from 7.5 to 8 and at temperatures from 350C to400C. The various divalent metal ion were tested, Ca++ and Zn++ were found to enhance the activity whereas Mg++, Cu++, Mn++ inhibit the enzyme activity. Also reducing agents and inhibitors were tested. Reducing agent such as Dithiothreitol (DTT) inhibit the enzyme activity whereas sodium sulphide, SDS increases the enzyme activity. 1,10phenanthroline and EDTA were increases the enzyme activity.
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