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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
PsbO is a ubiquitous extrinsic protein of photosystem II required for providing proper environment for water splitting activity of photosystem II. Exposure of wheat to heat stress initially reduces the rate of photosynthesis by disintegrating the photosystem II, primarily by disruption of the oxygen evolving complex (OEC). Here, we identified and cloned putative PsbOgene of 1 kb from wheat cv. HD2329 using de novo assembly. Expression analysis showed higher expression of PsbO transcript in HD2985, as compared to HD2329 during pollination and grain-filling stages. We observed significant variations in the expression of PsbO in response to differential HS. A positive correlation was established between the expression of PsbO and activity of antioxidant enzymes like superoxide dismutase, catalase and guaiacol peroxidase in response to heat stress in contrasting wheat cvs. at different stages of growth. Photosynthetic rate was observed slightly higher in thermotolerant cultivar at different stages, as compared to susceptible. There is, however, need to discover many more isoforms of PsbO from wheat in order to characterize their role in the modulation of carbon assimilatory pathway under heat stress. Maintaining the integrity of PSII under HS using PsbO will pave the way for the development of ‘climate-smart’ wheat.