National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The aim of the present study was to detect biofilm formation of Methicillin Resistant Staphylococcus aureus (MRSA) by two methods. One hundred forty two bacterial isolates were obtained from the core of (63) infected tonsils collected from Al-Basra General Hospital in Basra city, cultured on orientation chromagar.34 isolates were identified as Staphylococcus aureus, out of which 33 isolates were identified as MRSA by using CHROMagar MRSA. Biofilm formation of the 33 MRSA isolates were screened by Congo red agar (CRA) and iced gene detection. Thirty three isolates out of 34 isolates (97%) were identified asS. aureus, and 33 isolates (100%) as MRSA on CHROMagar media. The females (19 patients) were more than males (14 patents) with percentages (57.6%,42.4%) respectively. Twenty patients aged from (1-9 years), nine patients aged from (10-19 years),three aged from (20-29 years),and one patient aged from (30-39 years) represented (58.8%, 29.4%, 8.8% and 3%) respectively. Fourteen (42.4%) isolates showed a black crystalline morphology, indicating biofilm production was observed on Congo red agar and 33(100%) in molecular method by detection of icaD gene. The molecular methods gave the most accurate to determine the MRSA producing of biofilm compared routine methods.
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