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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 7, Issue:1, January, 2018

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com /
submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(1): 2227-2232
DOI: https://doi.org/10.20546/ijcmas.2018.701.268


Mass Production of Entomopathogenic Fungi (Metarhizium anisopliae) using Different Grains as a Substrate
S.V. Agale1*, S. Gopalakrishnan2, K.G. Ambhure1,Hemkant Chandravanshi1, Rajeev Gupta1 and S.P. Wani2
 
1Department of Entomology, Indira Gandhi Krishi Vishwavidyalaya, Raipur, 492012, Chhattisgarh, India
2International Crops Research Institute for the Semi-Arid Tropics (ICRISAT),Patancheru 502324, Hyderabad, Telangana, India
*Corresponding author
Abstract:

Investigations were carried out towards the “Mass production of entomopathogenic fungi (Metarhizium anisopliae) using different grains as a substrate” at International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru, Hyderabad during 2016-17. The accomplishment of microbial control of insect pests, the successful mass production of the microbial agents in the laboratory significant availability of the pathogen is a primary requirement in the biocontrol programme. Metarhizium anisopliae is an entomopathogenic fungus which is used against a number of insect pests’ management and very successful biopesticides in integrated pest management practices to reduces the cost of production and minimize the environment and public health hazard. To develop a proficient method for the deployment of this fungus as a bio control agent, various grains and liquid media such as Potato Dextrose Broth and Sabouraud’s Dextrose Broth were screened. Significantly highest conidial count (67.6 × 103 spores/ml) was observed on green gram followed by sorghum in 103 dilutions, the assessment fungus whereas SDB produced significantly higher spore production of the fungi. Highest conidial count (63.7 × 103 spores/ml) was observed on SDB media followed by PDB in 103.


Keywords: Metarhizium anisopliae, Grain substrates, Mass production

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How to cite this article:

Agale, S.V., S. Gopalakrishnan, K.G. Ambhure, Hemkant Chandravanshi, Rajeev Gupta and Wani, S.P. 2018. Mass Production of Entomopathogenic Fungi (Metarhizium anisopliae) using Different Grains as a Substrate.Int.J.Curr.Microbiol.App.Sci. 7(1): 2227-2232. doi: https://doi.org/10.20546/ijcmas.2018.701.268
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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